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Hollow Fibre Modules:

• MicroKros

• MidiKros

• MiniKros Sampler

• MiniKros Sampler Plus

• MiniKros

• MiniKros Plus

• KrosFlo

• KrosFlo Plus

• KrosFlo Max

ŧ CellFlo

• CellFlo Plus

• CellFlo Max

• KrosFlo SIP

 

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CellFlo Modules

Disposable Hollow Fibre Tangential Flow Modules for Cell Separations, 0.5 to 1000 L

Overview

Disposable CellFlo hollow fibre membranes are specially designed for the gentle and efficient separation of whole cells in microfiltration, bioreactor perfusion and culture harvest applications. CellFlo combines the advantages of tangential flow microfiltration (0.2 ĩm and 0.5 ĩm pores sizes) with larger hollow fibre flow channels (1 mm ID) to provide gentler efficacious micro-separations without the risk of cell lysis. Other cell separation technologies have a higher risk of lysis resulting in ruptured cells and culture harvests contaminated with intracellular macromolecules. Perfectly suited for continuous cell perfusion and bacterial fermentation, CellFlo membranes isolate secreted proteins while eliminating spent media containing metabolic wastes and drawing in fresh nutrient-rich media. Consequently, CellFlo enables cultures to grow to a higher cell density with higher viability providing as much as a ten-fold increase of daily production of secreted proteins.

Whether performing a cell perfusion or conducting a simple microfiltration, disposable CellFlo modules can either be autoclaved or purchased irradiated (irr) for quick sterile assembly. We offer disposable CellFlo membrane modules in the full range of MiniKros and KrosFlo sizes and surface areas for processing volumes ranging from 0.5 to 1000 litres. All CellFlo membranes have a 1 mm fibre inner diameter, available in 0.2 ĩm and 0.5 ĩm pore sizes.

CellFlo Modules
KrosFlo Pilot System

Applications

• Continuous cell perfusion
• Cell washing and/or harvesting
• Clarification of cellular debris for protein

• Bacterial fermentation harvesting
• Monoclonal antibody production
• Processing diagnostic micro-particles

Cell perfusion for labs Cell perfusion for production

Specification

Parameter Description
Housing Clear and pigmented polysulfone
Connections • 1/4" HB inlet/outlet ports with FLL filtrate ports;
• 1/2" HB inlet/outlet ports with FLL filtrate ports;
• 3" inch sanitary tri-clover (TC) inlet/outlet ports with 1.5" sanitary tri-clover(TC) filtrate ports
Potting Polyurethane
Process Volume 0.5 - 1000 L
Surface Area 500 cmē - 4.1 mē

Membranes Fibre ID MWCO
Mixed Cellulose Ester (ME) 1.0 mm 0.2 µm
Polyethersulfone (PES) 1.0 mm 0.2 µm, 0.5 µm
Polysulfone (PS) 1.0 mm 20 nm

Surface Area (m²) ID (mm) TL (cm) L (cm) EL (cm) Dimensions
  70 22.9 22.9 20.0
  70 46.7 46.0 41.5
  70 69.1 69.1 65.0

Please contact us for current prices, more technical details or assistance.

Phone (403) 287-2345 • Toll-free 1-(800)-565-2345 • Fax (403) 287-1355 • E-mail: sincaninc@telus.net

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