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Hollow Fibre Modules:

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MicroKros Modules

Hollow Fibre Modules for Small Volume Filtration and Characterization,  2 to 50 mL

Overview

Designed for gentle cross flow filtration of small volumes ranging from 2–50 mL, disposable MicroKros hollow fibre modules are the first efficient and practical tangential flow filtration device for R&D volumes in the laboratory setting. While MicroKros modules are typically operated by a peristaltic pump, these devices also offer the unique advantage of manual operation for quick and convenient separations for small volumes. Concentration utilizes two retentate syringes connected to the luer inlet/outlet ports to pass the sample back and forth through the membrane lumen while a third syringe collects the filtrate by way of one of the side-ports. In diafiltration, a fourth syringe can be used to either supply more sample for concentration or buffer for diafiltration via the luer T connector.

Applications

• Cell washing
• Lysate clarification
• Bacterial concentration
• Micro-particle diafiltration
• Macromolecular isolation

• Virus purifications
• Viral clarification
• Protein purification
• Antibody production
• Desalting and buffer exchange

Benefits

• 5 – 30 minute separations.
• Two modes: pump operation and manual operation.
• Gentle process for increased viability.
• 90 – 99% product purity.
• 85 – 95% product recovery.

Hand Operation

While MicroKros modules can be operated using a small capacity peristaltic pump, these devices also offer the unique advantage of hand operation for quick and convenient separations of very small volumes. Hand operation utilizes two retentate syringes connected to the luer inlet/outlet ports (by way of Luer T's) to pass the sample back and forth through the membrane lumen. A third syringe is connected to one of the female luer side ports to receive the filtrate as the retentate volume diminishes. A fourth syringe, connected to one of the inlet Luer T's can be used to either supply more sample for concentration or buffer for diafiltration.

Concentration Diafiltration

Specification

Parameter Description
Housing Polysulfone
Inlet/Outlet Ports Male Luer-Lok (MLL)
Permeate Ports Female Luer-Lok (FLL)
Potting Epoxy
Process Volume 2 mL - 60 mL
Surface Area 5 - 20 cmē
Packaging Dry, pre-wetted or irradiated

Membranes Fibre ID MWCO
Mixed Cellulose Ester (ME) 0.6 mm 0.1 µm, 0.2 µm
Polyethersulfone (PES) 0.5 mm 0.2 µm, 0.5 µm
Polysulfone (PS) 1.0 mm
0.5 mm
20 nm, 100 kD
0.05µm, 20 nm, 400 kD, 100 kD, 50 kD, 10 kD

Surface Area (cm²) ID (mm) TL (cm) L (cm) EL (cm) Dimensions
5-8 3.5 10.2 10.2 8.0
8-11 3.5 14.2 14.2 12.2
13-20 3.5 23.2 23.2 21.2

Please contact us for current prices, more technical details or assistance.

Phone (403) 287-2345 • Toll-free 1-(800)-565-2345 • Fax (403) 287-1355 • E-mail: sincaninc@telus.net

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